Shortening Lead-Times to Create an Agile

1. Introduction The last decade has witnessed a significant transformation that fast fashion has become a key feature in current fashion industry. Although it was regarded as a niche concept offered by a few winners such as Zara and H&M, fast fashion has now been adopted as a key operation mode by many brands in the fashion market (Baker, 2008), using advanced and more efficient supply chains to be more responsive to changing trends towards customer demand. Hence, Fast fashion brands perform relatively better than other fashion brands in various aspects. (Mattila et al. 002: 340 – 351) Esprit is a fashion brand owned by Esprit Holding Ltd. , manufacturing apparel, accessories, footwear and housewares under the Esprit label. In the previous fashion market, Esprit is generally recognized as the originator of fast fashion. As one of the pioneers in the fashion industry, however, Esprit has lost its way in recent years. (Best Retail Brands, 2012) â€Å"In essence, Esprit is a str ong and profitable brand, but the brand has gradually lost its soul over the past few years,† Ronald Van Der Vis – chief executive of Esprit admitted. News. com. au, 2011) The company is now focused on a brand revival with significant investment planned for marketing and the retail experience, as well as plans to exit some European markets and sell off the U. S. business. (Best Retail Brands, 2012) The most notable action is that the company has appointed Jose Manuel Martinez Gutierrez, who was group director of distribution and operations at Zara, as the CEO position in September 2012. This evidence shows that Esprit is going to struggle with fast fashion leaders like Zara and H&M.From an operational perspective, agile supply chain strategies have been relatively well studied, and can yield significant value to firms through faster supply chain. (Fisher and Raman 1996) Esprit has started realizing that how to make its products and services to reach customers in the sho rtest time is most important in today’s fashion market. As a result, rapidly changing competitive fashion market and dynamic customer expectations require Esprit to seek agility in its whole supply chain. This paper focuses on the brand reformation in the aspect of creating agile upply chain for Esprit, with an analysis of the key problem that Esprit is currently facing and provide three solutions, which are: 1. Designing new products following fashion shows 2. Establishing smaller-scale operational mode 3. Using quick response strategy 2. Literature Review 2. 1 The conception of fast fashion Fast fashion is a contemporary term used by fashion retailers to acknowledge that designs move from catwalk to store in the fastest time to capture current trends in the market. (Bruce et al. 001) In an operational level, Barnes and Lea-Greenwood (2006) defined â€Å"Fast fashion† as a business strategy which aims to reduce the processes involved in the buying cycle and lead times to get fresh fashion product into stores, in order to satisfy consumer demand. 2. 2 Agile supply chain in fast fashion The supply chain is central to the creation of fast fashion. If suppliers cannot respond quickly enough, inefficiency will occur, and clothing will be unsalable and finally result in the increase of inventory.Fashion market has been defined that it has four characteristics which are short life-cycles, high volatility, low predictability and high impulse purchasing (Martin et al. 2004), as a result, building up agile supply chain is highly essential in today’s fashion market. Bruce et al. (2004) has described agile supply chain that it is shorter, more flexible and demand driven than the normal supply chain. Christopher et al. (2004) pointed out that agile supply chain is driven by information such as market demand and information sharing between businesses in the supply chain. 2. 3 Three critical lead-timesAccording to Martin et al (2004), retailers should c oncentrate on using different ways to improve the quality of forecast to cope with various uncertainties from the market. However, because the vitality of market demands, the prediction of forecast will always be accurate in fashion market. As a result, the way of reducing lead-time should be found rather than just relying on the market forecasting. 2. 3. 1 Time-to-market In the highly changeable fashion industry, life cycles of fashion products have been reduced, which means that there is a need for firms to reduce the time-to-market of new products to get market at the fasted speed.Time-to-market is defined as the length of time taken in product development process from product idea to the finished product. (Garel, 2003) Griffin (2002) used time-to-market to analyze its relationship with the degree of product complexity and originality. In which product complexity and originality were found be related with multifunctional teams and the use of formal process. Sherman et al. (2002) presented that the quantity of people, such as suppliers, top management people and multi-functional teams who involved in the process is highly associated with time-to-market. It is also important to point out that Martin et al. 2004) has drawn a figure presenting the risk that companies will confront if slow to market. 2. 3. 2 Time-to-serve According to Martin et al. (2004), time-to-serve could be understood as the time to capture customer’s order and deliver the product to retail stores. Time-to-serve applies both principles of lean thinking and just-in-time process to time compression. However, it also inspects the incurring of costs and adding of value with proceeding time. This offers enterprises with a more comprehensive perception where progress can be made in order to reach a higher service levels concerning availability. Marcelo, 2011) Bergvall-Forsberg and Towers (2007) suggest that sourcing garments closer to consumer markets, particularly in Europe, could shorten the time-to-serve. 2. 3. 3 Time-to-react Time-to-react is one of the lead-times given by Martin et al. (2004), which means the time to adjust the output of the business in response to volatile demand. Because most supply chains are driven by ordering, understanding what customer actually request and do the quick response is a challenge that many fashion retailers are facing. (Martin et al. 2004) Forza and Vinelli 1997:125) has defined that â€Å"Quick response strategy acts on the single phases/activities throughout the entire chain-right from the producers of yarn up to the sales outlets-with the aim of reducing the time spans that elapse from the textile design stage to the purchasing of the garments by the final consumers†. Time-to-react can be accelerated through the tenet of quick response strategy, which has been divided into three parts: reducing excess stock from raw material to market, declining the risk of new product forecasting and speeding up the delivery. Birtw istle et al. , 2003) 3. Problem After decades of development, Esprit has become a mature, well-known and international fashion brand that offers customers various kinds of fashion products. However, as showed in the annual report of Esprit in 2011, the net profit of Esprit Holding Ltd. was 79 millions, has slumped sharply by 98%, compared with the same period last year. Moreover, Esprit has closed more than 100 physicals stores in the most areas of North America and some areas of Europe and China because its meager sales. (News. com. au, 2011)These situations directly result in the loss of customers and the damage of its brand image. In the current dilemma of Esprit, Weixiong Cheng, the executive vice-president of ME&CITY, considered that speed and flexibility are the most essential factors in this changeable fashion market, however, with conservative design and sluggish supply chain, Esprit failed to response to ever-changing market needs, whereas the leading fast fashion retailer such as Zara and H&M is struggling to continuously bring fresh fashion goods to customers, which have brought unprecedented competitive pressure to Esprit.Stephen and Aikaterini (2011) point out that because the fashion industry is characterized by intense and dynamic competition, participants are obliged to develop innovative structures and processes supporting market growth, maintaining competitive advantage and exploiting new product sectors and consumers. Martin et al. (2004) consider that the big gap between traditional fashion and fast fashion is the agility of the supply chain. Esprit puts too much emphasis on unique brand’s image, while ignoring the speed and flexibility in the market. The low-speed of updating new products can’t meet consumers demand.So it not only misses the best sales opportunities but also increases the risk of inventory. In this context, Esprit must find some ways to recover. 4. Solutions Martin et al (2004) explain that through effectivel y manage three critical lead-times; the competitive advantage of enterprises can be improved. They are time-to-market, time-to-serve and time-to-react. The speed of Esprit’s supply chain could be improved through these three aspects. 4. 1 Design new products following fashion shows The first solution is designing new products following recent fashion shows.A successful supply chain should be sensitive to the market and response to the product design as soon as possible. Compared with copy the design from other fast fashion brands, depending on fashion shows to design new products will be faster. So the time to design new products will affect the speed of fashion supply chain directly. Yinyin (2010) consider that â€Å"fast fashion† is a new term used to describe clothing collections that are based on the most recent fashion trends. It means the popular fashion factors will change rapidly which lead to the short life-circle of fashion products. The product is often ephe meral, designed to capture the mood of the moment: consequently, the period in which it will be saleable is likely to be very short and seasonal, measured in months or even weeks†(Martin et al. 2004). It requires that suppliers should grasp the fashion trends and design new products in short time. Fast fashion companies employ a large number of designers and buyers; they are responsible for collecting a complete range of fashion information and getting inspiration from fashion shows. Also, Hines and Bruce (2001:123) state that, the-term trend is closely related to future designs.The right decision should be made on the design and style of the clothing (Forza and Vinelli, 1997). If a company can catch the first opportunity to reach new market, it would be succeed in sales. With the advances in mass communication, consumers can easily keep up with the latest fashion trends (Liz and Gaynor, 2006:260). Mass consumers not just like â€Å"cheap†; in fact, they are more intere sted in â€Å"trendy and inexpensive. † For instance, easy chic, and short-term trend is often associated with the popular elements in a particular season, such as a particular color.Minxun (2012) points out that Esprit makes transition to be a fast fashion brand; the key point is product design that is not only new but also fast. It means Esprit should follow the fashion trends and meets the consumers’ demand to design products soon. In order to forecast the consumers taste, the Esprit designers should refer to some famous shows that contain the fashion ideas, trends and colors for the new season’s textile designs (David et al. , 2006). Esprit should not following other fashion brands, being the first one to entry new market. 4. Establish smaller-scale operational mode In traditional fashion companies, the total time in manufacture process is considerable which enormously lengthen the order to delivery cycle. The underpinning reason for this long manufacturing time largely lies in the batch-based production and shipping methods. Companies want to pursue cost-minimization and every step is quantities-processed separate from each other in the total manufacturing process. In previous years, about 80 percent of Esprit’s business in Europe is the wholesale rather than retail (Kailath T, 2002).This led to the European headquarters inclined resources to order-placing meetings. Esprit’s order-placing conferences from beginning two times a year became four times a year, until the final 12 times a year, and each time there was three months for lead-time (Kailath T, 2002). The traditional economic batch-quantities methods could truly minimize their costs of manufacturing as well as the costs of shipping. But this viewpoint of cost is too narrow and enterprises would ultimately lose their advantage competitiveness compared with those fast-fashion brands.Esprit needs to change its existing manufacture and shipping patterns urgently if th e company attempts to transform its existing operational system. It must abandon its conventional economies of scale principle both in manufacturing stage and shipping stage. Fast changing, small amount and a high variety of products are commonplace for fast fashion brands. The successful fast fashion brand ZARA, it remains about 30 percent of spare capacity rather than maximizing the use of its productivity (Andres M, 2003).ZARA gives up the pursuit of economies of scale and takes small quantities of production and distribution methods. Whether ZARA and H & M, an artificially created shortage of supply, not only helps cutting down products’ lead-times but also encourages consumers to buy the commodities quickly. Also, small-scale production increased flexibility of the production line in order to better respond to changes of customer needs. If Esprit wants to transform into a fast fashion brand, it must abandon its conventional bulk production style which just pursuing econo mies of scale.The company should cut down its outputs and increase the diversity of products. The adoption of lower output and higher variety of production could help Esprit drive higher service levels in terms of availability within the shortest possible time. Another critical problem in Esprit’s supply chain is its shipping time that largely delayed the time-to-serve. Company’s former underpinning philosophy of pursuing the economies of scale kept the costs of shipping down to some extent, but may ultimately self-defeating for missing the best sales opportunities and increasing the risk of inventory.The best way to solve this problem is to accelerate the commodities’ transport speed even though sacrifice some cost consideration. Fashion represents to be fast. Take successful fast fashion brand ZARA for example, for the sake that products can be shipped to regions outside of Europe within 48 hours. The company is willing to pay two percent of high airfreight co st price. In France, Germany, Italy, Spain and other EU countries, ZARA transport its products mainly by truck with an average of 36 hours to the chain stores.The sales in these areas occupy 70 percent of company’s total sales. As for the remaining 30 percent of sales, the company sends its products through airway to distant countries and regions such as Asia for the sake of improving the transport speed (Marcelo R, 2011). ZARA’s chain stores in China have their separate order rights the same as other stores all over the world, even a section only book one piece of clothing, ZARA could also guarantee to send it to the store within 48 hours (Marcelo R, 2011). If Esprit wants to reform ts operational system and earn much more profits in fashion market, the company must make efforts to curtail the products’ shipping time to gain competitive advantages compared with other fashion brands. What’s more, the shorter transporting time could help Esprit lower its s upply chain risk. 4. 3 Lead-time reduction by â€Å"Quick response† strategy Quick response strategy has been adopted by a growing number of fashion retailers in order to improve the company’s competitive advantage. Esprit makes sales forecast and then outsources to third parties half a year in advance.However, the market environment may change during this period; therefore, the lead-time must be shortened in order to risk reduction. Figure 1 Merchandise, data and financial transfer with quick response Source: taken from Quick response in retailing: components and implementation (1995, P. 13) As we can see from figure 1, Customers go to the store to purchase the products or services they need. The purchasing information will be collected, such as style, color, and number. Then this buying information would be sent to vendor for analyzing.Fernie (1994) suggests that the development of IT technology can ensure the implementation of the quick response strategy. For instan ce electronic data interchange (EDI). Suppliers manufacture products in accordance with the order requirements, transportation to retail stores through distribution channels. Esprit would be suggested to use the designs of their offshore suppliers. And Esprit buyers will make ultimate selects from these designs. Furthermore, the ultimate decisions making are based on analyzing historical sales data.In practice, celebrities, pop stars and fashion events have affected the design of the products. In this way, a large amount of money and time will be saved. Quick response strategy gives close attention to an effective and efficient delivery process of goods from suppliers to customers with minimum lead-time. It aims at the high profits, low risk, changing demand and attractive products. The short product life cycle, demand uncertainty and fierce competition are the characteristic of fashion industry. In this environment, market mediation is becoming more and more important. Fisher (1997 , P. 07) point out that an accurate prediction makes a good match between the demand and supply of the products. The ultimate goal of the implementation of the quick response strategy is to meet the customers demand. Therefore, Esprit Buyers and sales staffs should track and analyze recent sales data promptly, so as to recognize the best-selling styles and slow-moving style. For these best-selling items, buyers could inform the supplier’s design team to produce similar styles as quick as possible. For those slow-moving items, managers could take markdown or discount strategy, which could relieve the inventory pressures.In addition, when fashion companies choosing suppliers, the speed and flexibility should be taken into account, not just low cost (Fisher, 1997, P. 108). Esprit could combine local manufacturers with outsourcing to low-cost producers. More specifically, the high-tech products and core products could be manufactured in local factories. As for basic products, the y could be produced in low-cost regions. For example, the suppliers of North Africa provide trendy clothing within three weeks at a low price (Birtwistle et al. , 2003) Indeed, multi-sourcing is a good way to outsource.Furthermore, the relationships among stakeholders influence the effect of the implementation of quick response strategy. On one hand, they are independent of each other; on the other hand, they share information and support each other. Figure 2 Physically Efficient Versus Market-Responsive Supply Chains Physically Efficient ProcessMarket-Responsive Process Primary purpose Supply predictable demand efficiently at the lowest possible costRespond quickly to unpredictable demand in order to minimize stock outs, forced markdowns, and obsolete inventory Manufacturing focusMaintain high average utilization rateDeploy excess buffer capacity Inventory trategyGenerate high turns and minimize inventory throughout the chain Deploy significant buffer stocks of parts or finished go ods Lead-time focusShorten lead time as long as it doesn’t increase costInvest aggressively in ways to reduce lead time Approach choosing suppliersSelect primarily for cost and qualitySelect primarily for speed, flexibility, and quality Product-design strategyMaximize performance and minimize costUse modular design in order postpones product differentiation Source: taken from What is the right supply chain for your product? (Fisher, 1997, P. 08) It can be seen from this chart, buyers concerned about the procurement budgets, discount management and increased margins. Manufactures pay more attention to the volume of orders, production scale, as well as timing. For distributors, the new products must be picked and delivered to consumer precisely, cheaply and as quickly as possible (Birtwistle et al. , 2003). By this way, Esprit would achieve the purpose of inventory reduction and short lead-time, as well as fewer forecast errors. 5. Advantages and disadvantages AdvantagesDisadva ntages Design new products1.Occupy market share firstly and make profits 2. To be the market leader of fast fashion and gain consumer loyalty. 1. New products are risky because of the uncertain forecast. 2. The complex clothing manufacture lead to the high cost Establish smaller-scale operational mode 1. Decrease the lead-time in supply chain 2. Win more competitive advantages for the company 3. Decrease the risk of supply chain1. Lose the cost advantages that bought by the economies of scale 2. Lose part of the profits 3. Increased product ranges lead to the rising of cost Lead-time reduction by â€Å"Quick response† strategy . Reduce inaccurate forecasts 3. Helping to keep a safety stock 4. Sales and profits will be boost by quick response strategy 5. Consumers’ satisfaction will be improved1. To some extent, the product quality is ignored. 5. 1 Advantages 5. 1. 1 Advantages of designing new products First of all, short time-to-market leads to the sales increase. If a company can catch the first opportunity to reach new market, it would occupy the market share in the first time and be successful. Martin et al. (2004) consider that the supplier make the new products early will make greater profits than later.Otherwise, the early entrant makes much less obsolescent stock. Secondly, early entrant can to be the market leader and gain consumer loyalty. Gabszewicz et al. (1992) indicated that brand loyalty does confer considerable first-mover advantage, consistent with â€Å"hard to follow† results. If the Esprit can design the popular fashion clothing faster than others, it will be stand out in various fast fashion brands. After consumers admiring the fantastic design and amazing speed, it will replace Zara and become the leader of fast fashion. In long term, consumer loyalty is very important to against the strong competition. . 1. 2 Advantages of establishing smaller-scale operational mode In manufacturing stage, the company should cut down its outputs and increase the types of products. This practice could fundamentally decreases the lead-time in supply chain and wins competitive advantages in this rapidly changing fashion market. While in shipping stage, the adoption of some faster transporting ways such as airways and the establishment of a much quicker delivery system could not only shorten the lead-time but also improve delivery flexibility to cope with various changes.The shipping system will inevitably occur transportation breakdown due to some unexpected situations such as bad weather conditions. The traditional bulk transportation would unavoidably suffer huge losses when facing such cases. However, the smaller-amount modes of transportation can minimize such losses to a certain extent. 5. 1. 3 Advantages of quick response strategy Esprit will benefit lot from quick response strategy. Firstly, it can reduce inaccurate forecasts base on the previous sales data. Secondly, it could be very helpful to keep a safe ty stock. Thirdly, sales and profits will be boost by this strategy.Last but not least, consumer satisfaction will be improved. These benefits will enhance the competitive advantage. 5. 2 Disadvantages 5. 2. 1 Disadvantages of designing new products following fashion shows Shelby, H (2011) consider that the new product innovation is risky comes from the observation that only a fraction of new products become commercial success. Even though the new products are design depending on the consumers demand, it is hard to collect all the consumers’ hobby. On the other word, sometimes the survey data shows only represent a part of consumers’ taste.It is very difficult to make sure that others are willing to buy the new products. â€Å"It has been estimated, for instance, that between 60 and 90 percent of new products end their totally withdrawn or left unsupported† (Booz et al, 1968). So it has a high risk to be the number one or the fastest one which produce the new de sign clothing. Yen et al (2011) points that with the increasing number of new products introduced more frequently as well as the smaller volumes per product, the pool of skills required for clothing manufacturing is becoming more complex. It leads to high-cost as the result.For the different new design clothing, suppliers should change the supply chain frequently. That will cost a lot and can’t sell in high price that result in making little profit. 5. 2. 2 Disadvantages of establishing smaller-scale operational mode The solutions showed above in time-to-serve part are not without their inherent shortcomings. First of all, the company would lose its cost advantage that bought by the economies of scale. Then, because of the lower yields, company's production may not be able to meet the demand of the market. As a result, Esprit may lose this part of profits.Last but not least, company has to invest much more money to diversify company’s product range as well as create a much quicker transportation system. All these innovations would average up products’ costs ultimately. 5. 2. 3 Disadvantage of quick response strategy Quick response strategy emphasizes particularly on fast and effective. To some extent, the product quality is ignored. For instance, the multi-sourcing is difficult to guarantee uniform quality, and inevitably rejects into the market, that would lead to customer dissatisfaction and the frequently returns. 6. ComparisonSimilaritiesDifferences Design new products VS Establish smaller-scale operational mode 1. Shorten the lead-times 2. Average up the cost of products Different influence on risk Establish smaller-scale operational mode VS â€Å"Quick response† strategy 1. Speed up the distribution cycle 2. Improve consumers’ satisfaction 3. Decrease inventory risk Different influence on cost Design new products VS â€Å"Quick response† strategy 1. Boost sales and margins 2. Enhance the brand loyalty Different i nfluence on risk 6. 1Design new products VS Establish smaller-scale operational mode There are ome similarities between these two solutions. On the one hand, both of them aim at shorten the lead-times in the supply chain. On the other hand, the two solutions would average up the cost of products. The obvious difference between these two solutions is the influence on risk. The solution proposed in time-to-market part would increase the products’ risk, while in another solution, supply chain’s risk would be reduced. 6. 2Establish smaller-scale operational mode VS â€Å"Quick response† strategy The similarities between Time-to-serve and Time-to-react can be presented in three aspects.Fundamentally, these two solutions all speed up the distribution cycle that from manufacturing to the end-customers. Next, consumers’ satisfaction would be improved. Last, they all could help Esprit to decrease inventory risk. Their main difference lies in cost. Solution propose d in Time-to-serve section would cut down company’s cost while another one would increase it. 6. 3Design new products VS â€Å"Quick response† strategy The brand loyalty would be enhanced by these two methods. In addition to this, the sales and margins would be boosted. These advantages would make the company stand out in various fashion brands.As the mentioned former solution above that there is a hazard about the risky new products. Nevertheless, the later one lowers the risk of products management. 7. Conclusion In this paper, fashion supply systems are characterized by three critical lead-times: time-to-market, time-to-serve and time-to-react, which are highly essential to building up an agile supply chain. However, in the consideration of the current fast fashion industry and the today’s situation of Esprit, how to react to the target market in the right time is a priority that Esprit should mainly focus on.As apparel markets have become more varied and cha ngeable in the present retail environment, there is a transition from a production-driven to a market-driven approach in the fashion industry. (Bhardwaj, 2009) Hence, in order to survive, Esprit has to sustain competitive objectives by ensuring the brand meet the market demand. The quick repose strategy is a must for Esprit to equip it with agility to fight against the leading fast fashion brands at its first step. Reference Baker, R. (2008). Retail sector focus-fashion:following fast fashion. Marketing magazines , 37. Birtwistle, G. , Siddiqui, N. , & Fiorito, S. S. 2003). Quick response:perceptions of UK fashion retailers. International Journal of Retail & Distribution Management , pp. 118-128. 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Jenssen, Differential effects of the primary forms of cross functional integration on product development cycle time, Journal of Product Innovation Management, 17 (4), (2000), pp. 257-267 Bergvall-Forberg, J, and Towers, N. (2007), Creating agile supply networks in the fashion industry: a pilot study of the European textile and clothing industry, Journal of the Textile Institute, Vol. 98 Iss: 4, pp. 377-86 Vertica, B. and Ann, F. (2010), Fast fashion: response to changed in the fashion industry, The International Review of Retail, Distribution and Consumer Research, Vol. 20 Iss:1, pp. 165-173

1984 Oppression Essay

In the dystopian novel 1984, George Orwell portrays a society where betrayal is not only acceptable, but it is actively encouraged through the use of psychological manipulation, which demonstrates the weakness of the individual to resist oppression. The party has built the society in such a way that when Parson is turned in by his daughter to the Thought Police he reacts with â€Å"sort of a doleful pride† (233). Parson is an example of the unthinking masses of the society; he has been oppressed for so long his ability for individual thought has been greatly diminished. He doesn’t even question if he truly committed treason against Big Brother, he does not take into consideration that his seven year old daughter may be lying. Parsons doesn’t show any anger toward his daughter that betrayed him, his response is the exact opposite he is proud that his daughter is doing what is best for the party. The party’s oppression of the individual begins at birth, through methodical thought conditioning. The party transforms the children of the society into model citizens that are willing to betray their own flesh and blood. See more: Foot Binding In China essay Through the party’s use of psychological manipulation the framework of the society urges its citizens to pursue the enemies of Big Brother. This further cements the Party’s power. The party dehumanizes them so that their inhibitions are only showing loyalty to the party. Through the domination of thought the party ensures that its citizens are completely loyal to Big Brother. The telescreen is a tool for the party; the citizens of Oceania live in constant fear of being monitored by the screens that they end up betraying their thoughts through their body language. â€Å"The smallest thing could give you away. A nervous tic, an unconscious look, a habit of muttering to yourself-anything that carried with the suggestion of abnormality†(79). Oceania’s citizens don’t know when they are being watched, the byproduct of this is the citizens policing themselves, giving the Thought Police the ability to completely control them. The fear produced by the telescreens lead the people expose themselves to the eyes of the party, which ultimately leads them to betray themselves .The party overwhelms the minds capacity by a constant stream of propaganda coming out of the telescreen at all hours of the day allowing the party to completely dominate the minds of the people, taking away the potential of individual thought. Betrayal in the society George Orwell created in 1984 was expected. Through the domination of the individual thought, and their inability to resist that oppression, betrayal of one’s comrades was only natural. Individual thought was so greatly overshadowed by the party’s constant stream of propaganda in the novel that there was no place for it in the minds of the citizens. The psychological manipulation that the party inflicted on its citizens everyday made it impossible for the individual to resist oppression.

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LOVELY PROFESSIONAL UNIVERSITY CAPSTONE PROJECT REPORT TOPIC- ANTIMICROBIAL ACTIVITY OF DIFFERENT TYPES OF HONEY. PROJECT GUIDE- SUBMITTED BY- DR. AKSHAY GARG MOHIT KUMAR DEPT. OF BIOTECHNOLOGY REG. NO. – 10800037 ROLL NO- RB1R07B02 B. TECH BIOTECH. (8th sem. ) DATED- . 17-05-2012 CERTIFICATECertified that this project entitled â€Å"anti microbial activity of different types of honey † submitted by MOHIT KUMAR , students of biotechnology Department, Lovely Professioal University, Phagwara Punjab in the partial fulfillment of the requirement for the award of Bachelors of Technology (biotechnology) Degree of LPU, is a record of student’s own study carried under my supervision & guidance. This report has not been submitted to any other university or institution for the award of any degree.Date: 17/5/2012 Name of Project Guide Dr. Akshay Garg DECLARATION I, MOHIT KUMAR, student of B. Tech Biotechnology under Department of Biotechnology of Lovely Professional Unive rersity, Punjab, hereby declare that all the information furnished in this dissertation / capstone project report is based on my own intensive research and is genuine. This dissertation / report does not, to the best of my knowledge, contain part of my work which has been submitted for the award of my degree either of this niversity or any other university without proper citation. Date – 17/5/2012 Investigator- MOHIT KUMAR Regd. No. 10800037 Acknowledgement Any attempt at any level can't be satisfactorily completed without the support and guidance of learned people in my capstone projectâ€Å"Antimicrobial activity of different types of honey†.I would like to express my immense gratitude to my guide Dr. Akshay Garg for his constant support and motivation that has encouraged me to come up with this project. I also would like to thanks my group member who helped me in my project. MOHIT KUMAR ABSTRACT Honey is a traditional topical treatment for infected wounds. It can be effective on antibiotic-resistant strains of bacteria. Several local brands of honey(Dabur) collected from different sources is used in this study. Also the honey is collected from various apiaries from Pathankot(Punjab) and Saharanpur (Up).The bacterial culture were taken from the laboratory of the Lovely professional University and was revived in nutrient broth media and then sub-cultured in nutrient agar media. The method used to test this antibiotic sensitivity of honey was done with the help of Kirby Bauer method . Marked variations were observed in the antibacterial activity of these honey samples. Bacterial species used were Bacillus subtilis ,E. coli. , S. aureus and Burkholderia spp. The zone of inhibition produced against different bacteria using honey of different concentration are as follows: E. oli (pathankot sample): 100% = 2. 6cm, 75%= 1. 9cm, 50%= 1. 7cm,and 25%= 1. 2cm; E. coli (dabur sample): 100% = 2. 2cm, 75%= 1. 6cm, 50%= 1. 3cm, and 25%= 1. 1cm; E. coli (sahar anpur sample): 100% = 2. 8cm, 75%= 2. 4cm, 50%= 2. 2cm, and 25%= 1. 7cm; B. subtilis (pathankot): 100% = 3. 0cm, 75%= 2. 7cm, 50%= 2. 7cm, and 25%= 2. 5cm; B. subtilis (dabur): 100% = 2. 7cm, 75%= 2. 5cm, 50%= 2. 4cm, and 25%= 2. 2cm; B. subtilis (saharanpur):100% = 3. 2cm, 75%= 2. 7cm, 50%= 2. 3cm, and 25%= 1. 5cm; Burkholderia spp. (pathankot): 100% = 2. 1cm, 75%= 1. 9cm, 50%= 1. 6cm, and 25%= 1. 4cm; Burkholderia spp. dabur): 100% = 2. 4cm, 75%= 1. 7cm, 50%= 1. 5cm, and 25%=1. 2cm; Burkholderia spp. (saharanpur):100% = 2. 5cm, 75%= 1. 9cm, 50%= 1. 7cm, and 25%=1. 4cm; S. aureus (pathankot):100%=2. 1cm, 75%= 1. 7cm, 50%= 1. 4cm, and 25%=1. 2cm; S. aureus (dabur):100% =3. 0cm, 75%= 2. 7cm, 50%= 2. 2cm, and 25%=1. 8cm; S. aureus (saharanpur): 100% = 2. 9cm, 75%= 2. 5cm, 50%= 2. 0cm, and 25%=1. 6cm; Zones of inhibition of different treatment groups were measured by agar-well-diffusion assay and compared with control. The comparison of honey sample with distilled water control had pro ved it significant.CONTENTS PAGE 1. INTRODUCTION 7 1. 1) Honey as antibacterial agent 7 1. OBJECTIVE 9 2. LITERATURE REVIEWS 10 3. 1 TYPES OF HONEY 10 3. 2 COMPONENTS OF HONEY 11 3. MATERIALS AND METHODS 15 4. MATERIAL 15 4. 2 METHOD 16 4. RESULTS AND DISCUSSION 19 5. REFERENCES 32 1. ) INTRODUCTION Honey is a sweet food made by bees using nectar from flowers. The Honey is a sweet, viscous fluid produced by bees from the collection of nectar, primarily from flowers. It is considered to be a natural syrup. The Nectar is gathered by the bees and is slowly transformed into honey, through a long rocess involving the addition of enzymes and the gradual reduction of moisture. Honey is a rich source of carbohydrates mainly Fructose and Glucose. The chemical composition of honey varies depending on the plant source, season and production methods. Therefore the Colour, Concentration and Compounds vary depending on the floral sources. Other compounds which can be found in Honey include Pr oteins and acids such as Gluconic Acid (C6H11O7, also known as 2,3,4,5,6- pentahydroxyhexanoic Acid), Minerals and Anti-Oxidants such as Hydrogen Peroxide (H202) and Vitamins (B6 and B12), (BD.Yates et. al. 1996). Honey has a low pH and a low moisture content, which is usually on average about 17 percent. The Gluconic Acid in honey is produced when bees secrete Glucose Oxidase, while processing the nectar, this give honey a low pH. There are many varieties of honey from around the world which come in three main types which are liquid, whipped and comb. Several local brands of honey(Dabur) collected from different sources is used in this study. Also the honey is collected from various apiaries from Pathankot(Punjab) and Saharanpur (Up) .Each of these honey’s were chosen because they are organic and readily available in health food stores 1. 1) The Antibacterial Effects of Honey The honeys are shown to have antibacterial properties, in particular Manuka honey. Manuka Honey has had extensive research done on it. It has been shown in many studies that Manuka Honey has antimicrobial effects (Barret J. , et. al, 2005; Coumbes A. L. , et. al. 2004; Mundo, M A. 2004). The Mechanism of Antibacterial Properties Honey has many natural properties which enable it to inhibit bacteria.These properties include, a low pH which is in the range of pH 3. 2 to 4. 5, approximately 3. 9 which is due to its content of acidic compounds mainly Gluconic acid as stated above. A low pH is inhibitory to most bacteria. Since most bacteria live in environments around pH 7, the pH of honey could inhibit the bacteria (Barrett, J et. al. al 2005). This is because pH affects the way large proteins such as enzymes work. Which causes the shape of enzymes to change, which then alters the overall charge, this causes the protein to denature.Honey contains small amounts of Hydrogen peroxide (H202) this varies depending on the honey, it is produced as a result of the enzyme glucose oxidase activ ity in producing Gluconic acid (Mundo, MA. et. al. 2004). C6H12O6 + H2O + O2 > C6H12O7 + H2O2 (glucose oxidase reaction) When honey is used topically (as, for example, a wound dressing), hydrogen peroxide is produced by dilution of the honey with body fluids. As a result, hydrogen peroxide is released slowly and acts as an antiseptic. Hydrogen peroxide is a powerful oxidising agent (Free Radical) which has the ability to damage cells.In an aqueous solution hydrogen peroxide acts like an acid and can oxidise a variety of compounds, by accepting free unpaired electrons. This allows the formation of other free radicals, which then causes a cascade effect. Therefore altering biological structures and therefore damaging cells. Honey is primarily a saturated mixture of two monosaccharide, with a low water activity; most of the water molecules are associated with the sugars and few remain available for microorganisms, so it is a poor environment for their growth.If water is mixed with hone y, it loses its low water activity, and therefore no longer possesses this antimicrobial property. Honey consists of various constituents such as water, carbohydrates, proteins, vitamins, amino acid, energy and minerals. Besides the major ones, there must also be several minor constituents in honey, which may be playing a key role in determining the antimicrobial behaviour of honey. In the past, antimicrobial activity of honey had been reported only by using aqueous solution of honey.It is said that honey possesses antibacterial property but it is not clear whether it is the bulk honey or some fraction of it. The antibacterial properties of honey speed up the growth of new tissue to heal the wound. The bactericidal effect of honey is reported to be dependent on concentration of honey used and the nature of the bacteria Considering the fact that their might be some specific constituents which may be contributing to the antimicrobial behaviour, it was decided to carryout the studies u sing different solvents.The present study therefore deals with the constituents in the different solvents followed by evaluation of extract for their antimicrobial behaviour against certain species of bacteria. 2) OBJECTIVE Based on the above facts, the present study was defined with following objective: 1) To check the antibacterial effect of different honeys on. a. Gram-positive Staphylococcus aureus, b. Gram-negative Escherichia coli, c. Gram-positive Bacillus subtilis, and d. Gram- negative Burkholderia capicium 2) To check the effect of honey on bacterial strains using different-different dilutions. . 100% honey sample b. 75% honey sample c. 50% honey sample d. 25% honey sample 3) To compare the effect of honey and control (Distilled water) on a. Gram-positive Staphylococcus aureus, b. Gram-negative Escherichia coli, c. Gram-positive Bacillus subtilis, and d. Gram- negative Burkholderia capicium 3) LITERATURE REVIEWS Honey has been used with medicinal purposes since ancient tim es and there are reports of its topic use by Ayurvedic medicine, dating back to 2500 b. c. Hippocrates prescribed the use of honey for several indications, including treatment of wounds and gastritis.Its healing properties are mentioned in the Koran and in Bible. Honey is a part of traditional medicine for centuries. The antimicrobial activity of honey is due primarily to hydrogen peroxide produced enzymatically. However, in undiluted honey the acidity is also a significant antimicrobial factor. The pH, ranging from 3. 2 to 4. 5, is low enough to inhibit the development of pathogens, whose optimal pH range situates between 7. 2 and 7. 4. When applied to affected areas of tissue, acidity, osmotic effect and phytochemical factors decrease with dilution; however, the activity of hydrogen peroxide increases from 2. to 50 times. On these values, peroxide acts as an antiseptic, without causing cell damage. The composition of sugars in honeys, from different floral origins, inhibits the de velopment of various intestinal bacteria. All chemical and physical factors make the therapeutic properties of honey unique: fast decline of infections and healing of wounds, quick inflammation recovery, minimizing of wounds, stimulation of angiogenesis, as well as the development of epithelial and granular tissues. Honey to be used with topical medicinal purpose, certain requirements are needed, like being free f herbicides, pesticides, heavy metals and radioactive elements and sterilized to prevent secondary infections. 3. 1) TYPES OF HONEY – ON BASIS OF FLORAL SOURCE- Blended- Most commercially available honey is blended, meaning it is a mixture of two or more honeys differing in floral source, color, flavor, density or geographic origin. Polyfloral – Polyfloral honey, also known as wildflower honey, is derived from the nectar of many types of flowers. The taste may vary from year to year, and the aroma and the flavor can be more or less intense, depending on which bloomings are prevalent.Monofloral- Monofloral honey is made primarily from the nectar of one type of flower. Different monofloral honeys have a distinctive flavor and color because of differences between their principal nectar sources. To produce monofloral honey, beekeepers keep beehives in an area where the bees have access to only one type of flower. Honeydew honey- Instead of taking nectar, bees can take honeydew, the sweet secretions of aphids or other plant sap-sucking insects. Honeydew honey is very dark brown in color, with a rich fragrance of stewed fruit or fig jam, and is not sweet like nectar honeys. . 2) COMPOSITION OF HONEY Carbohydrates Unsurprisingly, these comprise the major portion of honey – about 82%. The carbohydrates present are the monosaccharide fructose (38. 2%) and glucose (31%); and disaccharides (~9%) sucrose, maltose, isomaltose, maltulose, turanose and kojibiose. There are also some oligosaccharides present (4. 2%), including erlose, theanderose and panose, formed from incomplete breakdown of the higher saccharides present in nectar and honeydew. Proteins and Amino Acids.Honey contains a number of enzymes, including invertase, which converts sucrose to glucose and fructose; amylase, which breaks starch down into smaller units; glucose oxidase, which converts glucose to gluconolactone, which in turn yields gluconic acid and hydrogen peroxide; catalase, which breaks down the peroxide formed by glucose oxidase to water and oxygen; and acid phosphorylase, which removes inorganic phosphate from organic phosphates. Honey also contains eighteen free amino acids, of which the most abundant is proline. Vitamins, Minerals and AntioxidantsHoney contains trace amounts of the B vitamins riboflavin, niacin, folic acid, pantothenic acid and vitamin B6. It also contains ascorbic acid (vitamin C), and the minerals calcium, iron, zinc, potassium, phosphorous, magnesium, selenium, chromium and manganese. The main group of antioxidants in hon ey are the flavonoids, of which one, pinocembrin, is unique to honey and bee propolis. Ascorbic acid, catalase and selenium are also antioxidants. Generally speaking, the darker the honey, the greater its antioxidising properties. Other compoundsHoney also contains organic acids such as acetic, butanoic, formic, citric, succinic, lactic, malic, pyroglutamic and gluconic acids, and a number of aromatic acids. The main acid present is gluconic acid, formed in the breakdown of glucose by glucose oxidase. Honey also contains hydroxymethylfurfural, a natural product of the breakdown of simple sugars below pH 5. According to studies done by Patricia E. Lusby et al Twelve of the 13 bacteria were inhibited by all honeys used in this study with only Serratia marcescens and the yeast Candida albicans not inhibited by the honeys.Little or no antibacterial activity was seen at honey concentrations 1%, with minimal inhibition at 5%. No honey was able to produce complete inhibition of bacterial g rowth. Although Medi honey and manuka had the overall best activity, the locally produced honeys had equivalent inhibitory activity for some, but not all, bacteria. He then postulated that honeys other than those commercially available as antibacterial honeys can have equivalent antibacterial activity. These newly identified antibacterial honeys may prove to be a valuable source of future therapeutic honeys. According to Peter C.Molan (2001), honey may be the â€Å"natural cure† for most bacterial infections that could replace man-made antibiotics. Researchers say that an enzyme in the honey turns into a tiny  amount hydrogen peroxide when combined with bodily fluids killing nearby bacteria. Honey also causes an increase in lymphocyte and  phagocyte activity (Cooper et al. , 2011). The honeys are shown to have antibacterial properties, in particular Manuka honey. Manuka Honey has had extensive research done on it. It has been shown in many studies that Manuka Honey has an timicrobial effects (Barret J. , et. al, 2005; Coumbes A. L. , et. al. 2004; Mundo, MA. 2004).In this study the antibacterial activity of the five honeys were compared, the Manuka honey was used as a positive control. The nonperoxide antibiotic activity is due to methylglyoxal (MGO) and an unidentified synergistic component. Most honeys contain very low levels of MGO, but manuka honey contains very high levels. The presence of the synergist in manuka honey more than doubles MGO antibacterial activity. In vitro antibacterial activity of raw and commercially available honey was tested against Gram-positive bacteria (Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa, Shigella spp. nd Salmonella spp. ). Both types of honey showed antibacterial activity against test organisms with the zone of inhibition ranging from 8. 13 to 30. 85 mm, while E. coli, S. aureus, and Shigella spp. showed sensibility towards both types of Honey. Both types of honey showed no effects on Salmonella spp. The potency of honey at 100%concentration was found to be higher than all other concentrations tested. However, no effect was observed at concentration of 6. 25% v/v honey in the case of both samples(Patricia E. Lusby 2004).To study the antimicrobial activity of honey, 60 samples of various botanical origin were evaluated antimicrobial activities against 16 clinical pathogens and their respective reference strains. The bioassay applied for determining the antimicrobial effect employs the well-agar diffusion method and the estimation of minimum active dilution which produces a 1 mm diameter inhibition zone. Streptococcus pyogenes,Bacillus cereus and Bacillus subtilis were proven to be up to 60% more resistant than their equal reference strains thus emphasizing the variability in the antibacterial effect of honey and the need for further research (C.Voidarou 2010). Natural products, either as pure compounds or as standardized plant extracts, provid e unlimited opportunities for new drugs because of the ready availability of chemical diversity (Cos et al. , 2006). Honey and lemon-honey are traditional remedies in the Middle East and China and for many centuries and have been used in the treatment and prevention of the common cold and various upper respiratory tract infections (Molan, 1992; Zulma ; Lulat, 1989). Antibacterial activity of the honeys was assayed using standard well diffusion methods. oncluded that the sandal wood from different sources and mixture of of – and -santanols were highly effective against gram negative bacteria including Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae and as well as yeast Candida albicans. The present work gives strong evidence of antibacterial activities of honey, sandal oil and black pepper (Sheikh Ahmad 2002). In general, all types of honey have high sugar content as well as low water content and acidity, which prevent microbial growth.Osmotic effect, effect of pH and hydrogen peroxide are represented as an â€Å"inhibition† factor in honey (Postmes et al. , 1993). Most types of honey generate hydrogen peroxide when diluted because of the activation of the enzyme glucose oxidase, which oxidizes glucose to gluconic acid and hydrogen peroxide (Schepartz and Subers, 1964). Hydrogen peroxide is the major contributor to the antimicrobial activity of honey,and the different concentrations of this compound indifferent honeys result in their variable antimicrobial effects (Molan, 1992).Moreover, non peroxide factors also play critical role. The content of non peroxide factors are related to the floral source and sometimes account for the major part of the antibacterial activity in honey(Molan and Russell, 1988). Forty samples of different honey types (Acacia, Ziziphus , Brassica and Citrus) were collected from different areas of Pakistan and analyzed for moisture, pH, total acidity, ash, electrical conductivity, hydroxyl methyl furfural (HMF), sucrose, total sugars, invert sugar, protein, proline contents as well as macro and micro elements.The variation in composition of honey samples was observed due to different types of flora. Likewise, a significant level (P ; 0. 05) of ash, electric conductivity, sucrose, total sugar as well as macro and micro elements was also found in these honey types. Different formulations of honey has significantly inhibited growth of pathogenic microorganisms, Staphylococcus aureus, Escherichia coli, Candida albicans and Aspergillus niger when compared to control group, which is an evidence that honey is a therapeutic agent being used since ancient time throughout the world. Feng et. al. 1994) The discrepancy in the observed antibacterial activity can be due to several reasons. One possibility might be related to the differences in susceptibility of each species of microorganism to the antibacterial activity of honey used. Similar observations are reported by others (Nzeako and Hamdi 2000; Ceyhan and Ugur 2001; Taormina et al. 2001). As reported by others (Melissa et al. 2004) dilution of honey enhances hydrogen peroxide mediated antibacterial activity may explain some of the discrepancies of observed with the antibacterial activity of these honey.The presence of unstable putative agents and/or thermolabile antibacterial agent(s) could also be inactivated during the experimental procedure and thus may be considered as possible explanation of the observed insensitivity of some honey samples found in the preliminary study. 4. ) MATERIALS AND METHOD 4. 1) Materials Several local brands of honey and Dabur honey collected from different sources is used in this study. Also the honey is collected from various apiaries from- Pathankot(Punjab) and Saharanpur (Up). In the antibacterial study, several bacterial species known to be pathogenic to human such as E. oli. , S. aureus and Burkholderia capicium and Bacillus subtilis was used. These strains were obtained from biote chnology lab. in accordance to given requirement. Glass wares used:- Conical flask (500 milliliter), Conical flask (100 milliliter), Petri plates, glass rod, test tubes spreader, glass beakers, Glass slides and cover slip, Plastic wares used:- Beakers, Conical flask (500 milliliter), Conical flask (100 milliliter), dropper, appendrof tubes (2ml), auto pipette tips (10? l, 100? l, and 1000? l) Instruments used:- Hot air oven, Autoclave machine, Laminar air flow hood, Incubator, Microwave ovenOther material used:- Sprit lamp, cork borer,test tube stand, auto pipette (10? l, 100? l, and 1000? l), parafilm wax,,filter paper Chemicals used:- Nutrient agar, nutrient broth, Mueller Hinton agar, 70% Methanol, 100% methanol, ethyl alcohol ,sodium chloride. 4. 2) Methods Physicochemical study: Appearance of different honeys was observed. pH is determined using conventional procedure like using ph scale. Antibacterial study: Antibacterial study was carried out in steps. In the first step, an i n vitro screening will be carried out using either disc diffusion or well diffusion method.Well diffusion was carried out using plate diffusion, which I preferred. Preparation of test materials: Test materials will be prepared by diluting each honey at different dilutions, 25 ? l/100 ? l, 50 ? l/100 ? l, 75 ? l/100 ? l and one with no dilution. Moreover, net honey was also used as test material. All dilutions were carried out with double distilled and deionised sterilized water. 3. 2. 1) Source of bacterial strains The E. coli. , S. aureus, Burkholderia capicium and Bacillus subtilis were revived from the stock available in the various Biotechnology laboratories of Lovely Professional University, Phagwara (Punjab), India.Sub-culturing of bacterial strains in nutrient broth:- 100ml nutrient broth was prepared and ten test tubes were taken, autoclaved, and after this the Bacterial strains was inoculated in the nutrient broth in different test tubes and then incubated for 24 hours in i ncubator . Sub-culturing of the bacterial strains on solid media:- For All Bacterial strains:- 5. 6g of Nutrient agar was added to the 200ml of purified water. Then solution was heated and boiled for 1 minute to completely dissolve the powder. Then media was autoclaved.Then media(10-15 ml) was poured in Petri plates. Then Bacterial strains were added to media. Then Petri plate were kept in incubator for 18-48 hours at 37 degree C. Saline preparation : Take . 58g of Nacl and dissolve it to 10ml of distled water and make saline before 15 min of spreading and take the colonies from the nutrient agar plates and mix properly in the saline. Nutrient agar medium for antimicrobial testing: 7g of nutrient agar medium was added to the 250 ml of distilled water and heated for 1 minute to complete dissolve medium then autoclaved .After that pouring the petriplates and left it 20 minute for solidify medium and make wells , after solidify put the different Honeys in the wells and kept it to incub ate for 18-48 hours at 37C. Testing of antibacterial activity using agar well diffusion method: The bacterial strains were inoculated into 10 ml of sterile nutrient broth, and incubated at 37  °C for 18 h. Each culture was then spreaded on the surface of sterile nutrient agar plate and also pour-plated in nutrient agar media to perform the test in triplet, one with the control and the other two ith the test sample. Making the wells With the help of a cork borer make wells in the agar carefully without tearing the gel. In two agar plate of all four sets, four wells were prepared with the help of sterilized cork borer. Then with the hep of micropipette put 100 micro litre of honey sample into the wellIn the wells of two plates of each set, samples of following concentrations: (1) 100% sterile Honey(2) 75% sterile Honey(3) 50% sterile Honey and (4) 25% sterile Honey; were added by using micropipette. Also in the a well water was added as negative control.Then with the help of micropi pette put 100 micro litre of honey sample into the well Replace the lid of the plate between putting sample into wells to minimize exposure to air-borne contaminants. Cover the lid tightly with paraffin tape to avoid contamination. Incubation of the plates . A temperature range of 35 °C  ± 2 °C is required for 24-48 hours. Do not incubate plates in carbon dioxide  as this will decrease the pH of the agar and result in errors due to incorrect pH of the media. Measuring zone of inhibition 1.Following incubation, measure the zone sizes  to the nearest millimeter  using a ruler or caliper; include the diameter of the well in the measurement . 2. All measurements were made with the unaided eye while viewing the back of the petri dish. Hold the plate a few inches above a black, nonreflecting surface illuminated with reflected light . 3. Record the zone size on the recording sheet. Figure1: Testing of antibacterial activity using agar well diffusion method 4. )RESULTS Sub-cultu ring of bacterial strains in nutrient broth:- Figure2: Culture E. coli. , S. ureus and Burkholderia capicium and Bacillus subtilis in Nutrient broth media. Sub-culturing of the bacterial strains on solid media:- Fig. –3 E. coli subcultured on nutrient agar Fig. – 4 B. Subtilis subcultured on nutrient agar Testing of antibacterial activity using agar well diffusion method: Fig 5 : No antibacterial activity seen as no honey is poured (taken as negative control) in which E. coli is grown Fig 6 : No antibacterial activity seen as no honey is poured (taken as negative control) in which Bacillus subtilis is grown ANTIMICROBIAL ACTIVITY OF VARIOUS HONEY’S AGAINST E. coliFIG. 7 – ZONES OF INHIBITION BY VARIOUS DILUTIONS OF HONEY(pathankot sample) IN E. coli: 100% = 2. 6cm, 75%= 1. 9cm, 50%= 1. 7cm,and 25%= 1. 2cm FIG. 8– ZONES OF INHIBITION BY VARIOUS DILUTIONS OF HONEY (dabur sample) IN E. coli : 100% = 2. 2cm, 75%= 1. 6cm, 50%= 1. 3cm, and 25%= 1. 1cm FI G. 9– ZONES OF INHIBITION BY VARIOUS DILUTIONS OF HONEY (saharanpur sample) IN E. coli: 100% = 2. 8cm, 75%= 2. 4cm, 50%= 2. 2cm, and 25%= 1. 7cm; ANTIMICROBIAL ACTIVITY OF VARIOUS HONEY’S AGAINST B. subtilis FIG. 10– ZONES OF INHIBITION BY VARIOUS DILUTIONS OF HONEY IN B. subtilis (pathankot sample): 100% = 3. cm, 75%= 2. 7cm, 50%= 2. 7cm, and 25%= 2. 5cm FIG. 11– ZONES OF INHIBITION BY VARIOUS DILUTIONS OF HONEY in B. subtilis (dabur sample): 100% = 2. 7cm, 75%= 2. 5cm, 50%= 2. 4cm, and 25%= 2. 2cm; FIG. 12– ZONES OF INHIBITION BY VARIOUS DILUTIONS OF HONEY in B. subtilis (saharanpur):100% = 3. 2cm, 75%= 2. 7cm, 50%= 2. 3cm, and 25%= 1. 5cm ANTIMICROBIAL ACTIVITY OF VARIOUS HONEY’S AGAINST Burkholderia capacium FIG. 13– ZONES OF INHIBITION BY VARIOUS DILUTIONS OF HONEY in Burkholderia capacium (pathankot sample): 100% = 2. 1cm, 75%= 1. 9cm, 50%= 1. 6cm, and 25%= 1. 4cm; FIG. 4– ZONES OF INHIBITION BY VARIOUS DILUTIONS OF HONEY Burkholderia capacium (dabur): 100% = 2. 4cm, 75%= 1. 7cm, 50%= 1. 5cm, and 25%=1. 2cm FIG. 15– ZONES OF INHIBITION BY VARIOUS DILUTIONS OF HONEY Burkholderia capacium (saharanpur):100% = 2. 5cm, 75%= 1. 9cm, 50%= 1. 7cm, and 25%=1. 4cm; ANTIMICROBIAL ACTIVITY OF VARIOUS HONEY’S AGAINST S. Aureus FIG. 16– ZONES OF INHIBITION BY VARIOUS DILUTIONS OF HONEY S. aureus (pathankot):100%=2. 1cm, 75%= 1. 7cm, 50%= 1. 4cm, and 25%=1. 2cm FIG. 17– ZONES OF INHIBITION BY VARIOUS DILUTIONS OF HONEY S. aureus (dabur):100% =3. 0cm, 75%= 2. 7cm, 50%= 2. 2cm, and 25%=1. cm FIG. 18– ZONES OF INHIBITION BY VARIOUS DILUTIONS OF HONEY IN S. aureus (saharanpur): 100% = 2. 9cm, 75%= 2. 5cm, 50%= 2. 0cm, and 25%=1. 6cm Appearance Appearance of each of honey brand was examined and it was observed that honey were brown to dark brown in color, whereas other honey were golden yellow in color. Dabur honey- golden colour,transparent Pathankot sample – golden colour wit h sufficient transparency Saharanpur sample – dark yellow colour with zero transparency The transparency is due to processing of honey,while less transparent samples are unprocessed and taken directly from bee hive.Table 1- Diameter of the Zone of Inhibition by different concentration of Pathankot honey sample on different Bacterial strains. Bacterial strains| Zone of Inhibition (in cm)| | Control(sterile DistilledWater)| 100%Honey sample| 75%Honey sample| 50%Honey sample| 25%Honey sample| E. coli. | 0. 0| 2. 6| 1. 9| 1. 7| 1. 2| Bacillus subtilis| 0. 0| 3. 0| 2. 7| 2. 7| 2. 5| S. aureus| 0. 0| 2. 1| 1. 7| 1. 4| 1. 2| Burkholderia capicium| 0. 0| 2. 1| 1. 9| 1. 5| 1. 4| Table2- Diameter of the Zone of Inhibition by different concentration of dabur honey sample on different Bacterial strains.Bacterial strains| Zone of Inhibition (in cm) | | Control(sterile DistilledWater)| 100%Honey sample| 75%Honey sample| 50%Honey sample| 25%Honey sample| E. coli. | 0. 0| 2. 2| 1. 6| 1. 3| 1 . 1| Bacillus subtilis| 0. 0| 2. 7| 2. 5| 2. 4| 2. 2| S. aureus| 0. 0| 3. 0| 2. 7| 2. 2| 1. 8| Burkholderia capicium| 0. 0| 2. 4| 1. 7| 1. 5| 1. 2| Table 1- Diameter of the Zone of Inhibition by different concentration of Saharanpur honey sample on different Bacterial strains. Bacterial strains| Zone of Inhibition (in cm)| | Control(sterile DistilledWater)| 100%Honey sample| 75%Honey sample| 50%Honey sample| 25%Honey sample| E. oli. | 0. 0| 2. 8| 2. 4| 2. 3| 1. 7| Bacillus subtilis| 0. 0| 3. 2| 2. 7| 2. 3| 1. 5| S. aureus| 0. 0| 2. 9| 2. 5| 2. 0| 1. 6| Burkholderia capicium| 0. 0| 2. 5| 1. 9| 1. 7| 1. 4| In the preliminary screening process was observed that some honey brands have more while some other have less antibacterial activity. Some honey showed antibacterial effect against E. Coli and some showed strongest activity against Bacillus subtilis ,S. aureus and Burkholderia capicium. Clear zones of inhibition were produced by concentrated without dilution.Through the analysis of average values of the diameters of each honey, it is possible to infer a possible pattern in which commercial honeys, such as DABUR honey, have a greater average diameter while the homemade honeys from different regions, have a lower average diameter even at less concentrations, but the best antimicrobial activity was shown by Saharanpur honey sample with was collected directly from the apiary without any processing and it showed clear and large inhibition zones for all bacterial strains which were used for this test.Though after the dilutions were made, the low concentrated samples of Saharanpur honey resulted in rather poor antimicrobial activity. The wells in which water was loaded shoed absolutely no antimicrobial activity which was used as negative control. The results also showed that lower concentration of honey made through dilution shows less antimicrobial activity than concentrated ones. Thus honey are definitely effective against the bacterial strains at all concentration s. 6) REFERENCES Bibi S, Husain SZ, Malik RN (2008). Pollen analysis and heavy metals detection in honey samples from seven selected countries. Pak. J. Bot. 40(2): 507-516 * Cooper R. A. , Molan P. C. , Harding K. G. (1999) Antibacterial activity of honey against strains of Staphylococcus aureus from infected wounds, J. R. Soc. Med. 92, 283–285. * Cooper R. How does honey heal wounds? In: Munn P, Jones R,editors. Honey and Healing. UK: International Bee Research Association; 2001. * de Jong H. J. 1999) The Land of Corn and Honey:The keeping of stingless bees (meliponiculture) in the ethno-ecological environment of Yucatan (Mexico) and El Salvador, Utrecht UniversityPress, Netherlands, 423 p. * Kamal A, Raza A, Rashid N, Hammed TG, Lami M, Gureshin MA,Nasim K (2002). Comparative study of Honey collected from flora of Pakistan. On Line J. Biol. Sci. 23(9): 626-627. * Khalil MI, Shahjahan M, Absar N (2006). Glycemic Response and Lycemic Index of Bangladeshi Honey in Type 2 Diabe tic Patients. Malaysian. J. Pharm. Sci. 4(1): 13–19. * Lusby PE, Coombes AL, Wilkinson JM.Bactericidal activity of different honeys against pathogenic bacteria. Arch Med Res 2005; 36: 464-467 * Molan P. C. (1997) The antibacterial activity of honey. The nature of the antibacterial activity,Bee World 73, 5–28. * Mundo MA, Padilla-Zakour OI, Worobo RW. Growth inhibition of foodborne pathogens and food spoilage organisms by select raw honeys. Int J Food Microbiol 2004; 97: 1-8. * Tumin N, Halim NA, Shahjahan M, Noor Izani NJ, Sattar MA,Khan AH, et al. Antibacterial activity of local Malaysian honey. Malaysian J Pharma Sci 2005; 3: 1-10.

Introduction to Organization Structure

Introduction to organizational structure An organization structure refers to an arrangement of people, relationship and responsibilities in carrying out company activities to achieve goal. In addition it also can define as how a people in the organization are group together and to whom they report. Formal structure is needed for larger organization that decisions have to be made about the delegation of various tasks. In an organization structure clearly indicate and separate between the work activities which define by their job role.In good organization structure should explain the relationship of authority who reports to whom and for managers, who reports to them. An organization structure usually illustrated graphically in an organization chart. There are several types of organization structure such as functional organizational structure, geographic organizational structure, matrix organizational structure and product organizational structure. Functional organizational structure re fers to a group of people in the organization or department that hold similar position and perform similar task. It also indicates that who reporting to whom.The traditional way to of the organization, this is organizing people by function. A Geographic organizational structure is typically found in companies with operation spread over a large region. This includes national and international office. It will have internal structure and management layers depending on each location. While matrix organizational structure combine two or more types of organizational structure usually is product organizational structure and functional organizational structure to create a unique blend of work environment. Product organizational structure is the departmentalization based on product and.Employee work in difference unit where by each unit holds the responsibilities of producing the product or service. This structure also known as a division structure. Base on this organizational structure ever y product structure will lead by division. Each division will responsible for each product. 1. 0 Functional Structure According to O’Toole. S â€Å"The term organizational structure refers to how the people in an organization are grouped and to whom they report. One traditional way of organizing people is by function. Some common functions within an organization include production, marketing, human resources and accounting†.Base on this definition we can say that in functional structure employee are grouped together into department and hold similar position also perform similar task. Chief Executive Director Human Resource Manager Finance Manager Account assistants Manufacturing Manager Engineering Manager HR officer FOL Manager EOL Manager Test Engineering Equipment Engineering HR Assistants Payroll officer Supervisor Supervisor Engineer Enigineer Appendix 1. 0: Sample of Functional Structure for Company ABC Referring to above chart, it clearly divided the task and re sponsibilities by function.Example for this company has four main functions which are finance, human resource, manufacturing and engineering. Every department has a difference roles and responsibilities. Finance department focus on accounting and payroll, where by manufacturing can concentrate on execution in order to deliver a good result by divided into two main focus which is FOL and EOL. Both were lead by area manager to ensure the efficiency of the result. For engineering department also separate into two main focuses, they are test and equipment engineering. Each 2 function plays a role in order to achieve the company goal.Base on their expertise the result given will be very fast, efficiently and effectively. 1. 1 Advantage of Functional Structure The advantage of functional structure is employee can communicate and share information easily as they are grouping together according to similarities in their position. Beside that escalation process also can be done fast as and ef fectively. In terms of management, especially manager it easy for them to monitor subordinate performance. Another benefit of functional organization structure is coherent chain of command. For example, referring to above chart Account and payroll fall under purview of Finance department.A clear chain of command is needed, this is because it creates standard operation procedures, besides established consequences also enhanced accountability. Another advantages of functional structure is it cans expedited decision making. This is because normally group decision making will slow down progress on project due to the diverse perspectives involved. But in this structure because people have similar professional and educational background the decision making process will be much easier. In addition functional structure also allows work to be done by qualified and skilled individual in the area concern.Hence, another advantage of this structure is that it reduces cost by reducing duplication and use of resource in the organization. 1. 2 Disadvantage of Functional Structure Functional structure also have disadvantage. The disadvantage of functional structure is because functional structure separate by function, as a result employee having a very little understanding or concern for other area instead of their own area. Because of this separation there is a barrier in communication and overall cooperation and coordination. Beside that in this structure individual focus is rather than a company focus. 3 2. Geographic Structure Geographic structure often use by large company that operate in many area. The main function of this structure is to co-ordinate the work and employee from difference unit and responsible for conducting business in certain geographic locations. Each location will have its own internal structure and management layers, allowing them to run the operation. Below is geographic structure for Open University Malaysia. From the chart Open University Malaysia has divided the organizational base on geographic which is northern region, southern region, and central region, east coast and Sabah and Sarawak.Each region will cover certain state in Malaysia. For Northern region it covered Perlis, Kedah, Pulau Pinang and Perak. For southern region represent Johor, Negeri Sembilan and Melaka. Kuala Lumpur and Selangor is in central region. For Kelantan, Terengganu and Pahang indicate as East Coast area. Last area is Sabah and Sarawak region that cover Sabah and Sarawak. Headquarters can easily monitor the performance of each area and might not need to go through every single state to check on the academic progress or activity happened at the particular centre. Open University MalaysiaNorther n region Perlis Kedah Pulau Pinang Souther n region Central region Kuala Lumpur Perak Negeri Sembilan Melaka Selangor East Coast Kelantan Terengganu Pahang Johor Appendix 2. 0 : Geographic Structure of Open University Malaysia 4 Sabah Sarawak Sabah Sarawak 2 . 1 Advantages of Geographic Structure The advantages of geographic structure are cost saving. By locating resource close to customer it will help organization to reduce the cost such as travelling expenses. Using a geographic structure allows companies to use local, experienced individuals to run the company’s operation.This is the most beneficial in international locations, where companies need help transitioning their operations to specific international markets. 2. 2 Disadvantages of Geographic Structure There are some disadvantages of geographic structure such as companies may find it difficulties to run this because it does not allow for centralized decision making. Another disadvantage is that it creates duplication of work also the use of the organization’s resource. Hence, due to the geographical area that located far from each other it will create the difficulties in coordinating between departments in organization. 3. 0 Matrix Structure Matrix structure refe rs to a hybrid structure that combines two or more departmentalization together at one time to create a unique blend of work environment. Usually matrix structure is a combination of product and functional structure. This structure created when there is an assignment or project need to carry out. In this structure employee are reporting to two difference supervisor or manager. This is the differences of matrix structure from other structure. Example is several engineer might be hired carry forth a similar function by pooling in their expertise.In addition, a single company producing two types of product with the same team in professional. For effective and smooth functioning, it is mandatory to share the information in matrix structure. Each project will have a project manager and difference team members will report to him. The matrix structure, if implemented effectively, may increase the ability to react the new customer demands. It also may potentially decrease lead times of new product. Usually employee involve in this structure have to report to 2 manager which is immediate manager and also project manager who is the person to monitor the progress of the project.When work is accomplished, the project team may get dissolved, and the workers from different functional areas may get reassigned to other project and task. Chief Executive Director Human Resource Manager Finance Manager Manufacturing Manager Engineering Manager Division of project manager FOL Manager Test Engineering manager Project manager Account assistants 1 Payroll officer 1 HR officer Account assistants 2 Payroll clerk HR Assistants Account clerk Supervisor 1 Supervisor 2 Engineer HR clerk Legends: The hilted boxes represent staff engage with project. Appendix 3. :Sample of Matrix Structure of Company ZZZ 6 Appendix 3. 0 represent matrix structure for company ZZZ. From the chart there is a project conduct by project manager from project department. To ensure this project success the project has involves staff from other functional structure such as finance, human resource, manufacturing and engineering. For this structure usually employee need to get agreement from immediate manager before they can proceed, this is because it will impact their regular job and employee also must has a strong commitment and dedicated to perform the task.There is an opportunity for them to learn a new thing that not in their job scope. The staff involve in this project will report to 2 manager which is project manager and their immediate department manager. Progress of the project will monitor by project manager while their immediate manager play a role to encourage the employee to perform the task with professionally. 3. 1 Advantages of Matrix Structure The advantages of matrix structure are allowing the organization to effectively and efficiently manage the project that is large scale and complex.The specialized employee can be chosen base on the merit of their work and functions that t hey carry forth. Involving in this structure will give chance to employee to success because of the work load and a lot of difference thinking forces is working on the project and therefore the stress, authority and problem solving skill become stronger. Another advantage of this structure is a cost saving. This is because the people working in the project is hired as a part one team also work under other teams.Meaning to say that the employee involve in the project will do the 2 difference job at one time. In addition this matrix structure will provide opportunity for both functional and product skill development. Thus it will reduce barriers between departments and increase the integration of functions. 3. 2 Disadvantage of Matrix structure Even though there are advantages of matrix structure, there is certain disadvantage as well. The disadvantages of this matrix structure are requiring higher level of management skill compare to other type of departmentalization.It will impact t he company in terms of increasing cost if there is a lot of project manager hired for the job. Another disadvantage of matrix structure is increased conflict levels. This structure also may cause participants to experience 7 dual authority, which can be confusing and frustrating. Very important in this structure is require participant to have a very good interpersonal skill. 4. 0 Product Structure Product structure define as an organization base on organizing employee and work in difference units, while each unit hold responsibility of producing a product or service.For example if company produce 4 difference products, there will have 4 difference divisions for these product. Below is a sample of product organization. In other words product structure divides the company into divisions that bring together those employee involved with a certain type of product or market service. Chief Executive Secretary Product Division Division A Beverage Division B Ice cream Engineering Division C Training Manufacturing Purchasing Division D Cereal Milk powder Infant 6 years plus 3 years plus Adult Appendix 4. 0: Sample of product Organization of Company XYZ.Referring to above chart from company XYZ can see that this company has produce 4 products which is beverage, ice cream, cereal and milk powder. For each product will have a difference division to control. Each division within a divisional structure contains all the necessary recourses and function within it. 8 4. 1 Advantages of Product structure The advantages of product structure is company has a better control for the product as each product has manage by difference division and each division can act as a separate profit centre. Hence, this structure also can create a positive competition between divisions.As every unit own and produce a difference product it will allow manager and employee to expend their expertise and experience that are related to the overall activity of the product. Beside that these structures al so allow management to evaluate the work performance of each division easily. Another advantage of this structure is, because of clear focus on market segment it help to meet customers’ needs. 4. 2 Disadvantages of Product Structure Product structure may also have some disadvantages. For example manager may only focus on their product to the exclusion of the rest of the organization.Due to each product having own functional area expert it may lead to increasing in cost. Beside that will have duplication in terms of process and material also will increase the company cost. Management may facing difficulties to coordinate across department also one of disadvantage of this structure. 5. 0 Conclusion In conclusion organization structure is important as it is a system that control and drive company performance to achieve goal. Difference structure has a difference functions, but the objective is the same which is to ensure company running with smooth and achieve the goal.Functiona l structure is a basic structure and it applies for all organization. It still relevant and can be use for any company and organization. Geographic structure is mostly use for multinational company which has many branches at other country. It also applicable for domestic company and organization such as Open University Malaysia. Matrix structure is a hybrid structure that required commitment and skill especially interpersonal skill to perform the task. For product structure basically is a organizing people base on the product.

Comparing the psyche of immigrants as depicted in the novels Call it Essay

Comparing the psyche of immigrants as depicted in the novels Call it Sleep By Henry Roth (1934) and Bread Givers by Anzia Yizierska (1925) - Essay Example But these historical migrations were triggered by single dimension grounds. As the modern era arrived into the scene it was found that the act of migration was the cause of a web structure that contained a multi strata formation and is far more complex than the previous historical migrations. This complexity developed in the minds of the immigrants themselves. This is particularly depicted in the novels "Call it Sleep" By Henry Roth written in 1934 and "Bread Givers" written by Anzia Yizierska in 1925. 'Bread Givers' written by Anzia Yezierska and 'Call It Sleep' written by Henry Roth each would give us unique perspectives on the lives of immigrants in the earliest years of the twentieth century. The comparisons between the two are quite interesting, as the contrasts would also be. The settings are similar; the families which are central figures in each book are far from that in the numbers of members and the areas they would come from. The texts enable the reader the ability to almost sense the differences and similarities between Sara Smolinsky and young David as they live their lives in the New York area. Comparing their life experiences and then contrasting their ideals of the life they would in America would indeed be intriguing. Call It Sleep exemplifies Henry Roth's fascination with modernist ... As a cultural portrait, Call It Sleep paints a vivid picture of immigrant life in early twentieth century New York, specifically that of the very large immigrant Jewish population. As a commentary upon the struggles of a minority group, Call It Sleep offers a poignant tale of a young boy and his often unsettling experiences both at home and in his community. On the contrary, Bread Givers, Anzia Yezierska's autobiographical novel, endures for the way it relates universal truths about the poverty and despair of new immigrants to America at the turn of the century. Within that universality, Yezierska's voice emerges as strong, female, and idiosyncratic as it reveals the particulars of her characters' inner lives. Like many of her female protagonists, Yezierska immigrated to New York's Lower East Side in her early teens. She was born in around Warsaw in the early 1880s - the exact year is not known. Her older brother Meyer had immigrated to America a few years earlier and had saved enough money to bring his parents and seven siblings to New York. Like many immigrants who passed through Ellis Island, Meyer was renamed, and Max Meyer set out to reinvent himself accordingly. Anzia, called Harriet Meyer when she joined him, later reclaimed her identity and took back her given name. "Bread Givers" can be regarded as a source of cultural and social history because, even though it is fictionalized, it gives rich details of life in the early 20th century and illustrates many social conditions. It should be noted that the book is not a primary history source, but rather a secondary source, full of facts that would be of immense value to get a notion of the insight and the psyche of an immigrant at that juncture of the period in American history. This book is the story of a young

You have chosen to return to college to finish your degree. Describe Essay

You have chosen to return to college to finish your degree. Describe reasons why you made this decision and persuade a friend to do the same - Essay Example In the first decade of the 21st century the United States lost 5.8 million manufacturing jobs (Americanmanufacturing, 2011). The jobs available in the US marketplace have shifted into the knowledge and service economy. In order to compete in the new job marketplace it is imperative to obtain a formal education at an accredited college or university. Without a college degree the only jobs that are available are minimum wage jobs and even those are becoming scarcer and companies are diluting those jobs by offering part-time instead of full-time employment. The unemployment rate in the United States has gone up during the last few years. The unemployment in the United States in July 2011 was 9.1% (Bls, 2011). Due to the fact that more people are unemployed the level of competition was gone up and people that have better credentials are getting the jobs in the marketplace. The most important educational credential employers seek in new recruits is a college education. I have realized that the only way for me to get a good job is to go back to school to earn a degree. A college education increases a person’s chances to get a job and the job obtained will have higher wages. A person with a bachelor’s degree will earn an extra $0.9 million over their lifetime in comparison to a high school graduate; while people with a master’s degree will earn $1.3 more than a high school diploma and $0.4 million more than a bachelor’s degree (Longley, 2011). The past constraints that sometimes hurt the ability of adults to obtain a college education no longer apply. For instance a working adult sometimes had the excuse that he could not go back to college because of employment obligations. Time used to be a variable that hurt a lot of working adults. Due to advances in technology there are solutions available for working adults. Online universities such as the

Prevention and Control of Juvenile Delinquency Research Proposal

Prevention and Control of Juvenile Delinquency - Research Proposal Example According to Hepburn and Goodstein (1986), criminal justice reforms are organization initiatives to develop programs that respond and reflect sociological perspectives on the development and prevalence of crime. Though reforms have been initiated mostly through social action, the implementations of reforms entail legislative or judicial action (Lupton, 1999; Lint, 2007). There are also a number of ways and modes for the advancement of reforms programs. In the United States, for example, criminal justice reforms trace its roots in the Pennsylvania Prison Society, considered as the original society-based or lobby group for penal reform in the country (Pillsbury, 1989). While in the case of the United Kingdom, criminal justice reforms were developed in legislative assemblies and were enforced as acts of parliament considered to be more political than social actions (Stenson & Sullivan, 2000; Parks, 2007). In the studies done by Gorton and Boies (1999) and Keith (2002), penal reforms are among the most sensitive to change and have historically been the focus of criminal justice reforms. However, recent trends have shown a greater emphasis on crime prevention, social collaboration in rehabilitation and integration (Squires, 2006). Increasing cost of criminal justice proceedings, penal systems, and rehabilitation programs as well researches emphasizing the rehabilitation of offenders have all contributed to the divergence from punitive regiments (Kemshall & Maguire, 2001). Hughes (1998; 2007) points out that this level of sensitivity and responsiveness is also dependent on changing trends in social perspectives and existing state capacity to implement criminal justice programs. He notes that the changes are not changes in held values but rather changes in the approach of providing a means to pursue offenders and deliver restitution to victims.